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本研究对来自世界不同稻区的29份亚洲栽培稻和7份野生稻过氧化物酶基因Os POX1编码区序列进行了分析。结果表明Os POX1编码区序列中存在6个变异位点,Tajima’s D检验表明符合中性进化规律,所有供试材料可归为六种单倍型。籼稻和粳稻均有优势单倍型,粳稻优势单倍型是H1,籼稻优势单倍型是H3,两个亚种在此位点上已发生明显的遗传分化;所有热带粳稻与温带粳稻共同享有单倍型H1,不存在明显的分化,Os POX1位点的变异与温度并不存在明显的关联;多数香稻(ARO)和AUS型品种具有H1单倍型,偏粳稻;4份O.nivara共享籼稻的优势单倍型H3,而3份O.rufipogon分别独享3个H4、H5、H6单倍型,与栽培稻不同,表明O.nivara与栽培稻亲缘关系更近,而与O.rufipogon较远。进化树分析表明H2单倍型与其他单倍型存在显著的差异。各单倍型编码蛋白并不存在差异,表明该基因功能在水稻进化中比较保守,与各水稻类型的分化形成无关。
In this study, the sequence of Os POX1 coding region of 29 Asian cultivated rice and 7 wild rice from different rice regions in the world was analyzed. The results showed that there were 6 variation sites in the sequence of Os POX1. The Tajima’s D test showed that it was in accordance with the law of neutral evolution. All the tested materials could be classified into six haplotypes. Both indica and japonica had the dominant haplotype, the dominant haplotype of japonica was H1, the dominant haplotype of indica was H3, and the two subspecies had obvious genetic differentiation at this locus; all tropical japonica and temperate japonica shared Haplotype H1, there is no obvious differentiation, Os POX1 site variation and temperature does not have a significant correlation; most fragrant rice (ARO) and AUS varieties with H1 haplotype, partial japonica rice; 4 O. nivara Shared the dominant haplotype H3 of indica rice, while 3 O. rufipogon had 3 H4, H5 and H6 haplotypes respectively, which was different from that of cultivated rice, indicating that O. nivara was more closely related to cultivated rice than O. officinalis. rufipogon farther. Phylogenetic tree analysis revealed significant differences between H 2 haplotypes and other haplotypes. The haplotype encoded proteins did not differ, indicating that the function of the gene is more conservative in the rice evolution, with the formation of each type of rice is not related to the formation of differentiation.