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采用透射电镜法对松材线虫的细胞自噬现象进行鉴认,并通过实时定量PCR的方法对不同毒力及不同发育期松材线虫的自噬基因BxAtg1和BxAtg8表达量进行测定。结果表明:饥饿诱导下不同毒力松材线虫存在细胞自噬现象,不同毒力松材线虫饥饿诱导12、24、36 h时,自噬基因BxAtg1表达量递减且趋势一致,而自噬基因BxAtg8在饥饿24 h时的强毒松材线虫体内表达量最高,在饥饿36 h时弱毒松材线虫体内表达量显著升高;与繁殖性松材线虫体相比,自噬基因BxAtg1和BxAtg8在扩散型3龄(JⅢ)和扩散型4龄幼虫(JⅣ)中的表达量均增加,推测自噬活性的调节机制可能与松材线虫的毒力相关,与松材线虫发育转型阶段的形态特征联系,暗示自噬可帮助松材线虫发育转型,从而提高松材线虫的适应性。
The autophagy of pine wood nematode was identified by transmission electron microscopy. The expression of BxAtg1 and BxAtg8 in pine wood nematode at different virulence and different developmental stages was determined by real-time quantitative PCR. The results showed that there was autophagy in different virulence of pine wood nematode induced by starvation. The expression of BxAtg1 decreased gradually and tended to be consistent with the induction of 12, 24 and 36 h of starvation in different virulence of pine wood nematode, while the autophagy gene BxAtg8 The expression of BxAtg1 and BxAtg8 in the virulent stage was significantly higher than that in the control group, and the expression of BxAtg1 and BxAtg8 was significantly higher than that of the control The expression of autophagy activity was probably related to the virulence of Bursaphelenchus xylophilus and the morphological characteristics of the stage of pine wood nematode development and transformation , Suggesting that autophagy can help pine wood nematode development and transformation, thereby improving the adaptability of pine wood nematode.