【摘 要】
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A modified method of preparing 20(S)-ginsenoside Rh2(G-Rh2) and the inhibitory effect of 20(S)-ginse- noside Rh2 on Hep-A-22 cells were investigated. The total
【机 构】
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College of Chemistry,Basic Medical School
论文部分内容阅读
A modified method of preparing 20(S)-ginsenoside Rh2(G-Rh2) and the inhibitory effect of 20(S)-ginse- noside Rh2 on Hep-A-22 cells were investigated. The total saponins and strong alkali were dissolved in glycerol at the atmospheric pressure, and the degradation was performed at a high temperature. After G-Rh2 had been isolated and purified, MTT(methyl thiazolyl tetrazolium) assay was applied to evaluating the effect of 20(S)-ginsenoside Rh2 on the cells viability and morphological changes were observed. It was shown that 20(S)-ginsenoside Rh2 can reduce Hep-A-22 cells viability in dose-dependent manner and the cells took on cell shrinkage, membrane blebbing, chromosomal condensations, especially under the higher concentrations of it. In conclusion, 20(S)-ginsenoside Rh2 can be prepared effectively that not only decreases viability but also induces the apoptosis of Hep-A-22 cells.
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