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前文已初步报道了天花粉蛋白的理化性质和N-端的部分氨基酸顺序.结晶天花粉蛋白经琥珀酸酐处理所得的琥珀酰天花粉蛋白,在88％甲酸中经溴化氰降解后的产物通过Sephadex G-75凝胶柱层析,可分得6个峰(见图1).
Physicochemical properties and N-terminal partial amino acid sequence of trichosanthin have been reported in the previous section Crystalline trichosanthin succinic anhydride treated succinyltrichine protein, which is degraded by cyanogen bromide in 88% formic acid, is purified by Sephadex G-75 Gel column chromatography, can be divided into 6 peaks (see Figure 1).