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In the uplink grant-free non-orthogonal multiple access(NOMA)scenario,since the active us-er at the sender has a structured sparsity transmission characteristic,the compressive sensing recov-ery algorithm is initially applied to the joint detection of the active user and the transmitted data.However,the existing compressed sensing recovery algorithms with unknown sparsity often require noise power or signal-to-noise ratio(SNR)as the priori conditions,which greatly reduces the algo-rithm adaptability in multi-user detection.Therefore,an algorithm based on cross validation aided structured sparsity adaptive orthogonal matching pursuit(CVA-SSAOMP)is proposed to realize multi-user detection in dynamic change communication scenario of channel state information(CSI).The proposed algorithm transforms the structured sparsity model into a block sparse model,and with-out the priori conditions above,the cross validation method in the field of statistics and machine learning is used to adaptively estimate the sparsity of active user through the residual update of cross validation.The simulation results show that,compared with the traditional orthogonal matching pur-suit(OMP)algorithm,subspace pursuit(SP)algorithm and cross validation aided block sparsity adaptive subspace pursuit(CVA-BSASP)algorithm,the proposed algorithm can effectively improve the accurate estimation of the sparsity of active user and the performance of system bit error ratio(BER),and has the advantage of low-complexity.