论文部分内容阅读
目的研究表明,Wnt信号通路与肾癌发生、发展密切相关。文中拟通过观察小分子Wnt信号抑制剂15-氧代绣线菊内酯对人肾癌786-0细胞增殖、迁移、细胞凋亡和周期等恶性表型的影响,探讨15-氧代绣线菊内酯对肾癌细胞的体外抗肿瘤效应。方法实验分为:低、中、高浓度15-氧代绣线菊内酯组(终浓度分别为1.25、2.50和5.00μmol/L的15-氧代绣线菊内酯)及对照组(DMSO),各组分别处理786-0细胞株,分别运用四甲基偶氮唑蓝(MTT)比色法和划痕损伤实验分别观察细胞增殖、迁移的变化,采用Annexin V-FITC/PI双染色及PI染色流式细胞术检测细胞凋亡及周期的变化情况。结果15-氧代绣线菊内酯能明显抑制肾癌786-0细胞株的增殖,半数抑制浓度为1.088μmol/L,降低细胞迁移距离(P<0.05);低、中、高浓度15-氧代绣线菊内酯组处理786-0细胞36 h后,细胞凋亡率[(12.17±0.56)%、(18.54±1.07)%、(50.74±1.28)%]较对照组[(7.85±0.42)%]明显升高(P<0.05),且随药物浓度增加,细胞凋亡率逐步升高。15-氧代绣线菊内酯能明显增加G2/M期的细胞,减少G0/G1期的细胞(P<0.05)。结论 15-氧代绣线菊内酯在体外细胞学水平能够显著抑制786-0细胞的增殖和迁移,诱导细胞凋亡,可能是一种潜在的抗肿瘤药物。
Objective Studies have shown that Wnt signaling pathway is closely related to the occurrence and development of renal cell carcinoma. This article intends to observe the small molecule Wnt signal inhibitor 15-oxyplitlinelactone on human renal cell carcinoma 786-0 cell proliferation, migration, apoptosis and cycle and other malignant phenotype, to explore 15-oxo Spiraea In Vitro Antitumor Effect of Chrysalacetate on Renal Cancer Cells. Methods The experiment was divided into 15-oxadimenolactone groups (15, 25, 25 and 15 μmol / L final concentration) ), 786-0 cells were treated in each group. The proliferation and migration of 786-0 cells were observed by MTT assay and scratch test respectively. Annexin V-FITC / PI double staining And PI staining flow cytometry to detect apoptosis and cycle changes. Results 15-Oxymeline reduced the proliferation of renal cell carcinoma 786-0 cells, the median inhibitory concentration was 1.088μmol / L, decreased the migration distance (P <0.05); low, medium and high concentrations of 15- Compared with control group [(7.85 ±)], the apoptotic rate of 786-0 cells treated with mesolactone for 24 h was significantly higher than that of control group [(12.17 ± 0.56)%, (18.54 ± 1.07)%, (50.74 ± 1.28)%] 0.42)%] (P <0.05), and with the increase of drug concentration, the apoptosis rate gradually increased. 15-oxo-mesolactone significantly increased G2 / M phase cells and decreased G0 / G1 phase cells (P <0.05). CONCLUSION: 15-Oxymelinernellactone can significantly inhibit the proliferation and migration of 786-0 cells and induce apoptosis in vitro cytology level, which may be a potential anti-tumor drug.