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目的研究姜黄素对气道平滑肌细胞(airway smooth mus clecells,ASMCs)增殖的作用。方法体外培养大鼠原代ASMCs。CCK-8法检测血小板源生长因子(platelet-derived growth factor,PDGF)和PDGF加姜黄素处理的ASMCs A450的光密度值,以观察PDGF诱导的增殖和姜黄素的抗增殖作用。Western blotting检测ERK1/2蛋白的表达水平。结果与对照组(1.04±0.12)比较,CCK-8法检测给予PDGF 2d后,10ng/mL PDGF组(1.16±0.14)和50ng/mL PDGF组(1.30±0.15)的A450的光密度值均显著增加,P<0.01。给予姜黄素处理12、24和36h后,与对照组比较,11.25μmol/L组(21.57%、43.55%和65.99%)、16.88μmol/L组(39.64%、57.44%和77.80%)和25.3μmol/L组(44.50%、53.68%和92.68%)的细胞平均抑制率均增加显著,P<0.05。姜黄素(25μmol/L)加PDGF(25ng/mL)处理20、40和60min后ERK1/2蛋白表达水平均显著降低。结论姜黄素对增殖的ASMCs有抑制作用,可能与抑制了ERK1/2的信号通路活化有关。
Objective To study the effect of curcumin on the proliferation of airway smooth muscle cells (ASMCs). Methods Rat primary ASMCs were cultured in vitro. CCK-8 assay was used to measure the optical density of platelet-derived growth factor (PDGF) and curcumin-treated ASMCs A450 to observe PDGF-induced proliferation and antiproliferative effect of curcumin. Western blotting was used to detect the expression of ERK1/2 protein. Results Compared with the control group (1.04±0.12), CCK-8 assay showed significant optical density values of A450 in 10 ng/mL PDGF group (1.16±0.14) and 50 ng/mL PDGF group (1.30±0.15) after 2 days of PDGF administration. Increased, P<0.01. After treatment with curcumin for 12, 24, and 36 h, compared with the control group, 11.25 μmol/L group (21.57%, 43.55% and 65.99%), 16.88 μmol/L group (39.64%, 57.44%, and 77.80%) and 25.3 μmol The average inhibition rate of cells in the /L group (44.50%, 53.68%, and 92.68%) increased significantly, P<0.05. Curcumin (25μmol/L) plus PDGF (25ng/mL) treatment significantly decreased ERK1/2 protein expression after 20, 40, and 60 min. Conclusion Curcumin can inhibit the proliferation of ASMCs, which may be related to the inhibition of ERK1/2 signaling pathway activation.