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目的:了解新疆经典型卡波氏肉瘤(Kapos’s sarcoma,KS)患者皮损、外周血单核细胞(peripheral blood mononuclear cells,PBMC)、唾液和尿液中人疱疹病毒型(HHV8)DNA病毒载量水平及分布情况,探讨HHV8病毒载量与经典型KS临床分期之间的相关性。方法:选取2011-03-09-2012-11-11就诊于新疆医科大学第一附属医院皮肤科8例经典型KS患者的4种类型32份样本中扩增HHV8DNA ORF26基因片段,应用RT-qPCR测定32份样本中HHV8病毒载量,评价载量负荷与经典型KS疾病临床分期之间的关系。结果:全部资料均获完整基因组DNA。新疆经典型KS患者不同样本中HHV8DNA检出率为100%。标准曲线良好,扩增产物特异,其中,HHV8病毒载量负荷皮损组织为205.75±124.02,唾液为70.75±53.01,PBMC为39.88±26.98,尿液为28.25±12.26,差异有统计学意义,F=11.206,P<0.001。HHV8病毒载量水平皮损组织>唾液>PBMC>尿液。结论:新疆经典型KS患者多样本中均有HHV8DNA检出,HHV8病毒载量水平在皮损中最高,在尿液中最低,与新疆经典型KS临床分期之间未呈现相关性趋势。
OBJECTIVE: To understand the histopathological changes of skin lesions, peripheral blood mononuclear cells (PBMCs), human herpesvirus (HHV8) DNA viral load in saliva and urine of patients with Kapos’s sarcoma (KS) Level and distribution, to investigate the correlation between HHV8 viral load and clinical stage of classic KS. Methods: The HHV8 DNA ORF26 gene fragment was amplified from 4 types of 32 samples from 8 patients with classic KS in the Department of Dermatology, the First Affiliated Hospital of Xinjiang Medical University from 2011-03-09 to November 11, 2011. The RT-qPCR The HHV8 viral load was measured in 32 samples to assess the relationship between load burden and clinical stage of classic KS disease. Results: All data were intact genomic DNA. Xinjiang KS samples of different samples of HHV8DNA detection rate was 100%. The standard curve was good and the amplification products were specific. Among them, the tissue of HHV8 viral load was 205.75 ± 124.02, saliva was 70.75 ± 53.01, PBMC was 39.88 ± 26.98, urine was 28.25 ± 12.26, the difference was statistically significant, F = 11.206, P <0.001. HHV8 viral load level Skin lesions> Saliva> PBMC> Urine. CONCLUSION: HHV8 DNA is detected in many samples of patients with classic KS in Xinjiang. The highest level of HHV8 viral load is found in lesions and the lowest in urine. There is no correlation between the level of HHV8 and the clinical stage of classic KS in Xinjiang.