一、方法原理利用光谱进行定量分析的基础是Beer-Lambert定律,即在单一组分体系中存在如下关系式: A=kC (1)其中A为吸光度,C为溶液浓度,k为比例常数。考虑到偏离Beer定律的情况,在上式中加入截距项修正: A=kC+k_0 (2)对于m个组分的体系,设有n个试样,测定的波长数为k,则可用矩阵形式表示为: First, the principle of the method Quantitative analysis using spectroscopy is based on the Beer-Lambert law, that is, in a single component system there is the following relationship: A = kC (1) where A is the absorbance, C is the concentration of the solution, k is the proportionality constant. Considering the deviation from Beer’s law, add the intercept term correction in the above equation: A = kC + k_0 (2) For a system of m components, with n samples, the number of wavelengths measured is k, The matrix form is expressed as: