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利用以炭纤维为载体的生物膜反应器处理酵母废水,通过逐步提高进料浓度的方式,对厌氧生物膜反应器处理高浓度有机废水的能力及反应器内微生物的多样性变化特征进行了探索。结果表明,当控制水力停留时间(HRT)为6d,进料COD浓度由启动时2 798mg/L分阶段提升至5 596和13 990mg/L的过程中,相应的沼气容积产气率分别为0.06、0.14和0.37L/(L·d),平均甲烷含量分别为57%、71%和74%,COD平均去除率分别为45%、64%和65%,反应器表现出了较强的抗有机负荷冲击能力。当进料COD浓度继续提高至22 980mg/L,对应COD容积负荷达4.7g/(L·d)时,COD去除率降至49%,但pH能维持在7.7,平均甲烷含量为66%,沼气容积产气率进一步上升到0.58L/(L·d)。可见,过高的进料容积负荷虽然提高了容积产气率,但会造成沼气转化效率的下降。高通量测序结果表明,随着有机负荷的增加和运行时间的延长,反应器内微生物菌群逐渐稳定并演化成了以甲烷螺菌属(Methanospirillum)、甲烷鬃菌属(Methanosaeta)、甲烷杆菌属(Methanobacterium)为古菌代表和以食酸菌属(Acidovorax)、芽孢八叠球菌属(Sporosarcina)为细菌代表的优势菌属构成的沼气微生物菌群,优势菌属占总微生物数量的比例为58%。启动初期和稳定期的沼液中古菌、细菌所占比例分别为69.23%、30.77%和66.86%、33.14%;产甲烷类菌株在古菌菌群中的比例从起始阶段的33.11%提升到稳定阶段的76.70%,氢营养型产甲烷菌成为古菌群落结构中占绝对优势地位的类群(由起始阶段的24.67%升高到稳定阶段的60.13%)。
By using biofilm reactor with carbon fiber as carrier to treat yeast wastewater, the biofilm reactor’s ability to treat high-concentration organic wastewater and the diversity of microorganisms in the reactor were improved by gradually increasing the feed concentration explore. The results showed that the corresponding biogas volume gas production rates were respectively 0.06 for HRT 6d and COD concentration from 2 798mg / L to 5 596 and 13 990mg / L during the start-up. , 0.14 and 0.37L / (L · d) respectively, the average contents of methane were 57%, 71% and 74%, respectively. The average COD removal rates were 45%, 64% and 65%, respectively. Organic load impact. When the feed COD concentration continued to increase to 22 980 mg / L and COD volume loading reached 4.7 g / (L · d), the COD removal rate dropped to 49%, but the pH was maintained at 7.7 with an average methane content of 66% Biogas volume gas production rate rose further to 0.58L / (L · d). It can be seen that although the high feed volume load increases the volumetric gas production rate, it will result in a decrease in the biogas conversion efficiency. The results of high-throughput sequencing showed that with the increase of organic load and the prolongation of running time, the microbial flora in the reactor gradually stabilized and evolved into the genus Methanospirillum, Methanosaeta, Methanobacterium Methanobacterium is a biogas microbial flora composed of archaebacteria and dominant bacteria represented by Acidovorax and Sporosarcina. The proportion of dominant bacteria in total microorganisms is 58%. The proportions of archaea and bacteria in the biogas slurry during initial and initial phases were 69.23%, 30.77%, 66.86% and 33.14%, respectively. The proportion of methanogens in archaebacteria increased from 33.11% at the initial stage to At 76.70% of the stable stage, hydrogenotrophic methanogens became the predominant species in the archaeal communities (from 24.67% in the initial stage to 60.13% in the stationary stage).