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以粳稻日本晴(O.sativa L.subsp.Japonica cv.Nipponbare)为受体和轮回亲本,以广西普通野生稻核心种质DP15为供体,通过连续回交和SSR标记辅助选择的方法构建普通野生稻单片段代换系群体(Single segment substitution lines,SSSLs)。用覆盖水稻全基因组的563对SSR引物检测供体(DP15)和受体(日本晴)的多态性,从中挑选212对分布在水稻12条染色体上的多态性引物跟踪供体基因型。结果表明,在BC3F1代获得79个代换片段,这些片段基本上能相互重叠并覆盖水稻12条染色体,其中第1、第2条染色体含有的代换片段数最多,均为9个,第9、第10和第12条染色体含有的代换片段数最少且均为5个。79个代换片段长度为12.65～113.55 cM,平均长度为54.3 cM,总覆盖长度为4285.28cM,是水稻整个染色体组长度的2倍多,覆盖野生稻全基因组达98.89%。随机选取其中的6个代换株系用96个多态性引物检测其遗传背景,发现供体DNA残留率为7.3%～15.6%。今后将继续通过回交和标记辅助选择,以获得一整套覆盖普通野生稻全基因组的SSSL库,这将有助于研究稻种的起源、演变和分化以及普通野生稻的功能基因组。
Japonica japonica (O.sativa L. subsub.Japonica cv. Nipponbare) as the receptor and the recurrent parent, using the common accession core accession DP15 as the donor, the common wild type was constructed by successive backcrossing and SSR marker-assisted selection Rice single segment substitution lines (SSSLs). The polymorphisms of donor (DP15) and receptor (Nipponbare) were detected using 563 pairs of SSR primers covering the whole genome of rice, and 212 polymorphic primers distributed on 12 rice chromosomes were selected to track the donor genotypes. The results showed that in the BC3F1 generation, 79 substitutions were obtained. These fragments could basically overlap each other and cover 12 rice chromosomes. Among them, chromosomes 1 and 2 contained the largest number of substitution fragments, both of which were 9 and 9 , Chromosomes 10 and 12 contained the smallest number of substitutions and both were 5. The 79 substitutional fragments ranged from 12.65 to 113.55 cM in length, with an average length of 54.3 cM and a total length of 4285.28 cM, which was more than twice the length of the whole genome of rice and covered 98.89% of the whole genome of wild rice. Six of the six substitutions were selected randomly to detect the genetic background with 96 polymorphic primers and found that the donor DNA residual rate was 7.3% ~ 15.6%. In the future, a complete set of SSSL repositories covering the genomes of O. soja will be continued through backcrossing and marker-assisted selection, which will help to study the origin, evolution and differentiation of rice species and the functional genome of O. japonica.