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目的评价滤膜法、多管发酵法以及酶底物法对氯化消毒损伤大肠杆菌的检测效果。方法以大肠杆菌E.coli K12为指示细菌,并利用氯化消毒技术制备消毒损伤细菌,分别采用修复培养法和GB/T 5750—2006《生活饮用水标准检测方法》推荐的滤膜法、多管发酵法以及酶底物法进行检测。结果修复培养法和多管发酵法均检出消毒损伤大肠杆菌,而滤膜法均未检出消毒损伤大肠杆菌;对于酶底物法,消毒5 min时样品消毒损伤大肠杆菌的检出率为0%~47.50%,消毒20 min所有样品均未检出消毒损伤大肠杆菌。与修复培养法比较,滤膜法和酶底物法消毒损伤大肠杆菌的检出率均较低,差异均有统计学意义(P<0.05)。结论多管发酵法可有效检测氯化消毒损伤大肠杆菌。
Objective To evaluate the effect of membrane filtration method, multi-tube fermentation method and enzyme substrate method on the detection of E.coli with chlorinated disinfection injury. Methods Escherichia coli E.coli K12 was used as indicator bacteria, and disinfection-damaged bacteria were prepared by chlorination disinfection technique. The methods of repair culture and GB / T 5750-2006 “Standard Test Method for Domestic Drinking Water” Tube fermentation method and enzyme substrate method for testing. Results The disinfection-damaged Escherichia coli was detected by both the repair culture method and the multi-tube fermentation method, while the disinfection-damaged Escherichia coli was not detected by the membrane filtration method. For the enzyme substrate method, 0% ~ 47.50%, disinfection 20 min All samples were not detected disinfection injury Escherichia coli. Compared with the repair culture method, the detection rate of the E. coli with both the membrane filtration method and the enzyme substrate method was lower, the differences were statistically significant (P <0.05). Conclusion The multi-tube fermentation method can effectively detect the chlorination disinfection injury Escherichia coli.