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目的:观察含不同羟乙基淀粉(HES)溶液的血清对人肺腺癌A549细胞粘附、侵袭、迁移行为的影响。方法:择期行肺癌根治术患者30例,随机分为2组(n=15):6%HES200/0.5溶液组、6%HES130/0.4溶液组。麻醉诱导前于30min内给两组患者分别输注15mL/kgHES200/0.5和HES130/0.4溶液。输注前和输注结束后抽取静脉血10mL分离出血清。人肺腺癌A549细胞接种于培养板,随机分为4组(n=15):HES200/0.5血清组(HES200组)、HES130/0.4血清组(HES130组)、输注溶液前血清组(HF组)、正常对照组(C组)。HES200组、HES130组、HF组均以10%血清浓度与A549细胞孵育4h,C组不接受血清处理。采用细胞黏附实验检测细胞黏附率,Transwell法检测侵袭细胞数、细胞划痕实验检测细胞迁移率的变化,ELISA检测MMP-2、MMP-9表达水平。结果:与C组和HF组比较,HES200组和HES130组的细胞粘附率、侵袭细胞数、迁移率降低(P<0.05);HES200组的细胞黏附率、侵袭细胞数、迁移率低于HES130组(P<0.05)。与C组和HF组比较,HES200组和HES130组的MMP-2、MMP-9表达降低(P<0.05)。HES200组的MMP-2、MMP-9表达低于HES130组(P<0.05)。结论:含HES200/0.5和HES130/0.4溶液血清可能通过下调MMP-2、MMP-9水平抑制A549细胞黏附、侵袭和迁移行为,其中HES200/0.5血清作用较强。
Objective: To observe the effect of serum containing different hydroxyethyl starch (HES) on the adhesion, invasion and migration of human lung adenocarcinoma A549 cells. Methods Thirty patients undergoing radical resection of lung cancer were randomly divided into 2 groups (n = 15): 6% HES200 / 0.5 solution group and 6% HES130 / 0.4 solution group. Two groups of patients were infused 15 mL / kg HES200 / 0.5 and HES 130 / 0.4 solution respectively within 30 min before induction of anesthesia. Pre-infusion and infusion after the withdrawal of venous blood 10mL serum was isolated. Human lung adenocarcinoma A549 cells were seeded on culture plates and randomly divided into four groups (n = 15): HES200 / 0.5 serum group (HES200 group), HES130 / 0.4 serum group (HES130 group) Group), normal control group (C group). HES200 group, HES130 group and HF group were all incubated with A549 cells for 4h at 10% serum concentration. Group C was not treated with serum. The cell adhesion rate was detected by cell adhesion assay. The number of invasion cells was detected by Transwell assay. The cell migration was detected by cell scratch assay. The expression of MMP-2 and MMP-9 was detected by ELISA. Results: Compared with C group and HF group, the cell adhesion rate, invasion cells and migration rate decreased in HES200 group and HES130 group (P <0.05). The cell adhesion rate, invasive cell number and migration rate in HES200 group were lower than those in HES130 group Group (P <0.05). Compared with C group and HF group, the expression of MMP-2 and MMP-9 in HES200 group and HES130 group decreased (P <0.05). The expression of MMP-2 and MMP-9 in HES200 group was lower than that in HES130 group (P <0.05). CONCLUSION: The serum containing HES200 / 0.5 and HES130 / 0.4 may inhibit the adhesion, invasion and migration of A549 cells by down-regulating the levels of MMP-2 and MMP-9, of which HES200 / 0.5 serum is more effective.