By means of indirect immunofluorescence (IIF), indirect immunofluorescence-flow cytometry (IIF FCM) and Western blot, the gene expression level and localization and distribution of CenpG in HeLa-Tet Off cell with the inhibition of the exuorescence is much less; (2) CenpG expresses less; (3) the CenpG antigenicity is inhibited by around 79%. It is suggested that the effects of antisense CenpB onCenpGare highly consistent to that on its own sense CenpB gene, disclosing the rather close relation between these two centromere proteins.