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目的:研究不同加工方法对猪苓药材质量的影响,为建立该药材的合理产地加工方法提供参考。方法:采用晒干、阴干、不同温度烘干共10种加工方法处理猪苓药材,参照2015年版《中国药典》中水分、折干率、总灰分、酸不溶性灰分含量测定方法测定;利用苯酚-硫酸法显色,紫外-可见分光光度法(检测波长625 nm)测定猪苓总多糖含量;运用HPLC测定麦角甾醇的含量,流动相甲醇,检测波长283 nm。结果:不同加工方法处理的猪苓药材性状差异较小,有效成分含量存在显著性差异,40℃以上烘干处理获得的猪苓折干率较大,水分含量较小;40,50℃烘干处理时总灰分和酸不溶性灰分含量较低,麦角甾醇和总多糖含量较高。结论:综合分析药材有效成分含量、生产成本等因素,猪苓产地加工方法以低温40,50℃烘干法为宜。
Objective: To study the influence of different processing methods on the quality of Polyporus umbellatus and to provide a reference for establishing a reasonable processing method for its origin. Methods: 10 kinds of processing methods were used to treat the medicine of Polyporus umbellatus with 10 kinds of drying methods such as drying, drying in the sun and drying at different temperatures. The determination was made according to the method of determination of moisture content, drying rate, total ash and acid insoluble ash content in “Chinese Pharmacopoeia” The content of total polysaccharide in Polyporus umbellatus was determined by sulfuric acid method and ultraviolet-visible spectrophotometry (detection wavelength was 625 nm). The content of ergosterol was determined by HPLC. The mobile phase was methanol and the detection wavelength was 283 nm. Results: There was no significant difference in the traits of Polyporus umbellatus treated by different processing methods, and there were significant differences in the contents of active ingredients. The drying rate of Polyporus umbellatus obtained by drying at 40 ℃ was higher and the moisture content was smaller. Drying at 40 and 50 ℃ The total ash and acid-insoluble ash contents were lower, and the contents of ergosterol and total polysaccharide were higher. Conclusion: Based on the comprehensive analysis of the active ingredient content and the production cost, the processing method of Polyporus umbellatus was optimized by drying at low temperature of 40 and 50 ℃.