喉鳞状细胞癌(laryngeal squamous cell carcinoma,LSCC)是第二个最常见的头颈部肿瘤。它是以两个细胞周期监管者——CDKN2A和TP53频繁的畸变为特点的。然而,喉鳞状细胞癌常常作为头颈部癌症的一部分被研究,而不是作为一个单独的实体。在目前的研究中,我们的目的在于检查108位喉鳞状细胞癌病人中CDKN2A和TP53基因的突变状态。从新鲜的冰冻肿瘤组织中萃取DNA,通过直接测序筛查外显子1-3 CDKN2A和外显子5-8 TP53 Laryngeal squamous cell carcinoma (LSCC) is the second most common head and neck cancer. It is characterized by frequent aberrations of both the cell cycle regulators, CDKN2A and TP53. However, laryngeal squamous cell carcinoma is often studied as part of head and neck cancers rather than as a separate entity. In the present study, our aim was to examine the mutation status of CDKN2A and TP53 genes in 108 patients with laryngeal squamous cell carcinoma. DNA was extracted from fresh frozen tumor tissue and exon 1-3 CDKN2A and exon 5-8 TP53 were screened by direct sequencing