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为了解胰高血糖素及葡萄糖在动脉粥样硬化中所起的作用,运用细胞培弄手段,对伴和不伴高脂血清培养的兔主动脉平滑肌细胞进行细胞形态学和细胞动力学研究,结果显示:(1)不同浓度胰高血糖素均引起一定程度的细胞蜕变,但蜕变程度无剂量依赖性,而5%高脂血清复合胰高血糖素可造成细胞明显的增殖反应,形态学上表现为大量线粒体、核糖核蛋白体增生,多量幼稚胶原纤维生成。(2)不同浓度葡萄糖均引起细胞增殖抑制,抑制程度与葡萄糖浓度成正比(相关性检验r=-0.79,P<0.01);高脂血清复合葡萄糖对细胞增殖影响亦如单纯葡萄糖组(相关性检验r=-0.59,P<0.01),可见葡萄糖与高脂血清对平滑肌细胞增殖的影响无正的协调作用。上述结果提示:高脂血清伴血浆胰高血糖素浓度增高,可促进动脉粥样硬化的形成和发展,高浓度葡萄糖能引起平滑肌细胞的损伤。
In order to understand the role of glucagon and glucose in atherosclerosis, cell morphology and cell dynamics of rabbit aortic smooth muscle cells cultured with and without high-fat serum were studied by cell culture. The results showed that: (1) Glucagon at different concentrations all induced a certain degree of cell degeneration, but the degree of metamorphosis was dose-dependent. However, the combination of 5% high-fat serum and glucagon resulted in significant cell proliferation. Morphologically The performance of a large number of mitochondria, ribosomal protein hyperplasia, a large number of naive collagen fibers. (2) Different concentrations of glucose caused cell proliferation inhibition, and the degree of inhibition was proportional to glucose concentration (r = -0.79, P <0.01). The effect of glucose-induced hyperlipidemia on cell proliferation was also similar to that of simple glucose group Test r = -0.59, P <0.01), showing that glucose and hyperlipidemic serum smooth muscle cell proliferation without a positive role in coordination. These results suggest that: high-fat serum with elevated plasma glucagon concentrations can promote the formation and development of atherosclerosis, high concentrations of glucose can cause injury of smooth muscle cells.