角质形成细胞(keratinocyte,KC)受损可引起多种皮肤疾病,尖锐湿疣(condyloma acuminatum,CA)即为其中危害较大的一种,且近年感染率呈逐渐上升的趋势。CA皮损KC的培养方法各有不同。从人皮肤中分离KC进行体外培养已有30多年的历史,但由于培养条件和培养方法不同,使结果差异较大。因此,建立一种操作简便、成本低廉、细胞生物活性优良的人KC分离培养方法对CA的治疗具有重要意义。本文对CA患者的皮损KC的研究进展作一综述,为CA的治疗研究提供参考。 Keratinocyte (KC) damage can cause a variety of skin diseases, condyloma acuminatum (CA) is one of the more harmful one, and in recent years the infection rate showed a gradual upward trend. CA lesions KC different methods of culture. KC has been isolated from human skin in vitro for more than 30 years, but the results vary greatly due to different culture conditions and culture methods. Therefore, the establishment of a simple, low cost, excellent cell biological activity of KC separation and culture methods for the treatment of CA is of great significance. This review summarizes the research progress of KC in the lesions of CA patients and provides a reference for the treatment of CA.