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目的 :建立血浆中布比卡因反相高效液相色谱测定法 ,为临床监测血药浓度提供方法学基础。方法 :采用液 液萃取法并选用利多卡因为内标 ,以KromasilC18柱 ,流动相为甲醇 水 二乙胺 冰醋酸 (30 0∶10 0∶0 .4∶0 .2 ) ,紫外 2 30nm检测。结果 :本法在 0 .0 5～ 4 .0 μg·ml-1范围内线性良好 (r =0 .9998,n =6 ) ;最低检测限为 2 0ng(S/N≥ 3) ;绝对和相对回收率分别为(91.0± 2 .2 ) %和 (98.1± 1.0 ) % ;日内和日间精密度都小于 4 %。结论 :本方法简便可行 ,快速可靠
Objective: To establish a method for the determination of bupivacaine in plasma by RP-HPLC and to provide a methodological basis for the clinical monitoring of plasma concentration. Methods: Liquid-liquid extraction and lidocaine were used as internal standard. Kromasil C18 column was used. The mobile phase consisted of methanol, water, diethylamine and glacial acetic acid (30 0:10 0:0. 4:0. 2). Results: The method showed good linearity (r = 0.9998, n = 6) in the range of 0.05 ~ 4.0 μg · ml-1 and the lowest detection limit was 20 ng (S / N ≥ 3) The relative recoveries were (91.0 ± 2.2%) and (98.1 ± 1.0)%, respectively. The intra- and inter-day precision was less than 4%. Conclusion: This method is simple and feasible, fast and reliable