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[目的]观察肠吉泰对内脏高敏大鼠电压门控钠通道(VGSC)Nav1.8和神经生长因子(NGF)的影响。[方法]40只新生SD大鼠随机分成正常组、模型组、肠吉泰A组和B组4组;用醋酸刺激法建立内脏高敏大鼠模型;肠吉泰A组和B组分别每日予肠吉泰30g/kg和50g/kg灌胃,正常组和模型组予等量去离子水灌胃;4周后,采用直肠气囊扩张法评估内脏敏感性;取L6～S2脊髓背根神经节和直肠组织,采用RT-PCR和ELISA法分别检测Nav1.8 mRNA和NGF含量。[结果]与正常组相比,模型组大鼠内脏敏感性增高(P<0.01),Nav1.8 mRNA和NGF含量均明显增高(P<0.05);肠吉泰B组与正常组相比,内脏敏感性、Nav1.8 mRNA和NGF均差异无统计学意义;与模型组比,P<0.01或P<0.05。[结论]高剂量肠吉泰可能通过降低肠道NGF和脊髓背根神经节Nav1.8来降低内脏敏感性。
[Objective] To observe the effects of gaiji on voltage-gated sodium channel (VGSC) Nav1.8 and nerve growth factor (NGF) in visceral hypersensitive rats. [Methods] Forty neonatal SD rats were randomly divided into four groups: normal group, model group, gutta-percha A group and group B; rat model of visceral hypersensitivity was established by acetic acid stimulation; group A and B Gutta-perfused with Jiutai 30g / kg and 50g / kg orally, the normal group and model group were given the same amount of deionized water gavage; 4 weeks later, the rectal balloon dilation method to assess the visceral sensitivity; take L6 ~ S2 spinal cord dorsal root nerve Section and rectum. The contents of Nav1.8 mRNA and NGF were detected by RT-PCR and ELISA respectively. [Result] Compared with the normal group, the visceral sensitivity of the model group was significantly increased (P <0.01) and the content of Nav1.8 mRNA and NGF were significantly increased (P <0.05). Compared with the normal group, Visceral sensitivity, Nav1.8 mRNA and NGF were no significant difference; compared with the model group, P <0.01 or P <0.05. [Conclusion] High-dose Gutai may reduce visceral sensitivity by decreasing intestinal NGF and Nav1.8 in the dorsal root ganglion.