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目的了解Galectin-7在哮喘患儿支气管上皮细胞凋亡中的作用。方法经纤维支气管镜获得哮喘与非哮喘对照儿童的支气管黏膜,采用组织块培养方法进行支气管上皮细胞的培养,将RSV感染支气管上皮细胞,应用Micro array分析哮喘与非哮喘对照儿童支气管上皮细胞在RSV感染后表达有差异的基因;经GeneBank查询确定,这些差异基因中与凋亡有关的基因为Galectin-7。随后选取10例哮喘儿童和17例非哮喘对照儿童的支气管黏膜,应用原位末端标记法(TUNEL法)、原位杂交和免疫组化方法,分别对支气管上皮细胞凋亡、上皮细胞中Galectin-7及其mRNA表达进行观察。结果哮喘儿童支气管上皮细胞在RSV感染后Galectin-7基因上调8倍,哮喘儿童较非哮喘对照儿童支气管上皮细胞中Galectin-7以及mRNA表达增加,凋亡也同时增加。结论Galectin-7可能与哮喘患儿支气管上皮细胞的凋亡有关。Galectin-7可能通过这一作用,参与哮喘发病。
Objective To investigate the role of Galectin-7 in the apoptosis of bronchial epithelial cells in children with asthma. Methods Bronchial mucosa of asthmatic and non-asthmatic children were obtained by bronchofibroscopy. Bronchial epithelial cells were cultured by tissue culture method. Bronchial epithelial cells were infected with RSV. Microarray was used to analyze bronchial epithelial cells in children with asthma and non-asthmatic control. After infection, the differentially expressed genes were identified. GeneBank found that the genes involved in apoptosis in these differentially expressed genes were Galectin-7. Subsequently, bronchial mucosa of 10 asthmatic children and 17 non-asthmatic children were selected for bronchial mucosa. TUNEL, in situ hybridization and immunohistochemistry were used to detect the apoptosis of bronchial epithelial cells and the expression of Galectin- 7 and its mRNA expression were observed. Results The bronchial epithelial cells of asthmatic children were up-regulated by 8-fold after GV infection. The expression of Galectin-7 and mRNA in bronchial epithelial cells of asthmatic children increased and the apoptosis increased at the same time. Conclusion Galectin-7 may be involved in the apoptosis of bronchial epithelial cells in children with asthma. Galectin-7 may play a role in the pathogenesis of asthma.