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本实验通过高速逆流色谱从紫甘薯茎叶中制备分离得到绿原酸(1)、4,5-O-咖啡酰基奎宁酸(2,异绿原酸C)、3,5-O-咖啡酰基奎宁酸(3,异绿原酸A)及3,4-O-咖啡酰基奎宁酸(4,异绿原酸B)等四个高纯度天然化合物,并以4-硝基酚-α-D-吡喃葡萄糖苷(PNPG)为底物分别测定了四个化合物及紫甘薯茎叶提取物对α-葡萄糖苷酶的抑制作用。结果显示上述4个化合物及紫甘薯茎叶粗提物抑制α-葡萄糖苷酶的IC50值分别为:7.556μg/mL、1.419μg/mL、0.209μg/mL、9.339μg/mL和5.371μg/mL,均远远低于阳性对照阿卡波糖的IC50(355.4μg/mL),而进一步的酶抑制反应动力学分析结果显示化合物及提取物对α-葡萄糖苷酶表现为竞争性抑制类型。试验表明紫甘薯茎叶提取物具有显著的α-葡萄糖苷酶抑制作用,为甘薯茎叶资源作为降糖保健品或药品开发提供理论依据。
In this experiment, chlorogenic acid (1), 4,5-O-caffeoylquinic acid (2, iso-chlorogenic acid C), 3,5-O-caffeine Four high purity natural compounds such as acyl quinic acid (3, isocrochloric acid A) and 3,4-O-caffeoylquinic acid (4, isocrochloric acid B) α-D-glucopyranoside (PNPG) as substrates, respectively. The inhibitory effects of four compounds and purple sweet potato stem and leaf extract on α-glucosidase were measured. The results showed that the IC50 values of the four compounds and purple sweet potato stem-leaf extract inhibiting α-glucosidase were 7.556μg / mL, 1.419μg / mL, 0.209μg / mL, 9.339μg / mL and 5.371μg / mL , Were far lower than the IC50 of positive control acarbose (355.4μg / mL), and further enzyme inhibition reaction kinetic analysis showed that the compounds and extracts exhibited competitive inhibition of α-glucosidase. Experiments show that purple sweet potato stem and leaf extract has a significant inhibitory effect of α-glucosidase, sweet potato stem and leaf resources as hypoglycemic health products or drug development to provide a theoretical basis.