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目的:采用反相高效液相色谱法同时测定明目颗粒中淫羊藿苷和仙茅苷的含量。方法:采用C18色谱柱(4.6 mm×250 mm,5μm),以甲醇(A)-1.2%冰乙酸(B)为流动相进行梯度洗脱。梯度洗脱程序为:0~10 min,8%~15%A;10~15 min,15%~25%A;15~25 min,25%~30%A;25 min~50 min,30%A。检测波长为283 nm,柱温30℃,流速1.0 m L/min,进样量:10μL。结果:淫羊藿苷在进样量为0.02~0.18μg范围内线性关系良好,Y=0.595X+1.17,r=0.999 5;仙茅苷在进样量为0.04~0.36μg范围内线性关系良好,Y=0.614X+2.737,r=0.999 5。淫羊藿苷和仙茅苷平均加样回收率符合含量测定的要求。结论:该方法准确、简单、灵敏,可用于明目颗粒中淫羊藿苷和仙茅苷的含量测定。
Objective: Simultaneous determination of icariin and curculigin in Mingmu Granules by reversed-phase high performance liquid chromatography. Methods: C18 column (4.6 mm × 250 mm, 5 μm) was used for gradient elution with methanol (A) -1.2% glacial acetic acid (B) as mobile phase. The gradient elution program was: 0-10 min, 8-15% A, 10-15 min, 15-25% A, 15-25 min, 25-30% A, 25-50 min, 30% A. The detection wavelength was 283 nm, the column temperature was 30 ℃, the flow rate was 1.0 m L / min and the injection volume was 10μL. Results: Icariin showed good linearity in the range of 0.02-0.18 μg with Y = 0.595X + 1.17 and r = 0.999 5. Curculigo had good linearity in the range of 0.04-0.36 μg , Y = 0.614X + 2.737, r = 0.999 5. Icariin and curculigoside average recovery rate in line with the determination of the content of the test. Conclusion: This method is accurate, simple and sensitive and can be used for determination of icariin and curculigin in Mingmu granules.