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通过PCR技术成功克隆1个香蕉1,5-二磷酸核酮糖羧化/加氧酶小亚基基因的全长序列,并登录GeneBank,登陆号为FJ973633。利用生物信息学方法,分析香蕉1,5-二磷酸核酮糖羧化/加氧酶小亚基基因序列,对其推导的氨基酸的理化性质、亲水性/疏水性、跨膜结构、导肽、二级结构进行预测,并对香蕉1,5-二磷酸核酮糖羧化/加氧酶小亚基的氨基酸做进化发育分析,为进一步了解香蕉1,5-二磷酸核酮糖羧化/加氧酶小亚基在香蕉光合吸收中的作用奠定基础。
The full-length sequence of a ribulose-1,5-bisphosphate carboxylase / oxygenase small subunit gene was successfully cloned by PCR and deposited on GeneBank with accession number FJ973633. The bioinformatics methods were used to analyze the ribulose-1,5-bisphosphate carboxylase / oxygenase small subunit gene sequence of banana, and its deduced amino acid physical and chemical properties, hydrophilicity / hydrophobicity, transmembrane structure, Peptide, secondary structure of the predicted and banana 1,5-bisphosphate carboxylase / oxygenase small subunit amino acid evolution analysis, in order to further understand the banana 1,5-bisphosphate ribulose carboxylate The role of chemical / oxygenase small subunits in banana photosynthetic absorption lays the foundation.