一、实验动物:选健康家兔四只,每只体重2.5kg 左右,每组2只共分2组。二、样品制备:取原油用吐温80生理盐水配制成乳化剂,配成二种浓度,原油5%0.5%装入安瓿,100℃煮沸30分钟无菌。三、方法与剂量:1.用消毒过的1毫升注射器,注射1毫升乳化剂于家兔的左股四头肌中,右股四头肌注 1. Experimental Animals: Four healthy rabbits were selected, each weighing about 2.5 kg, and each group was divided into 2 groups. Second, the sample preparation: Take crude oil formulated with Tween80 normal saline emulsifier, dubbed two concentrations, crude oil 5% 0.5% into the ampoule, 100 °C boiled for 30 minutes sterile. Third, methods and doses: 1. With a sterile 1 ml syringe, injection of 1 ml of emulsifier in the left quadriceps of rabbits, right quadriceps feces injection