鉴于鼠麻风杆菌在鼠足垫(MFP)细胞中成功的传代已维持5年以上,作者企图用MFP细胞作人麻风杆菌的细胞培养。首先研究了MFP细胞的吞菌作用。将麻风杆菌混悬于含10％小牛血清的Eagle基础培养基中,浓度为2×10~7/毫升。将5毫升菌悬液标本接种于数个50毫升培养瓶的单层生长的MFP细胞上,各取一份培养于25、31、33和37℃,培养72和96小时后吸去培养基,并用温缓冲盐水充分冲洗细胞层以除去未被吞噬的菌。以后再将细胞用胰酶消化并 In view of the successful passage of M. leprae in mouse footpad (MFP) cells for more than 5 years, the authors attempted cell cultures of M. leprae with MFP cells. First studied the phagocytosis of MFP cells. Leprosy was suspended in Eagle’s basal medium containing 10% calf serum at a concentration of 2x10-7 / ml. Five milliliters of bacterial suspension samples were seeded on monolayer grown MFP cells in several 50 ml flasks, each taken at 25, 31, 33, and 37 ° C, and the medium was aspirated after 72 and 96 hours of culture, And rinsed the cell layer thoroughly with warm buffered saline to remove the non-phagocytosed bacteria. Later cells were trypsinized