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目的:探讨端粒酶在人类常见恶性肿瘤癌组织和癌旁组织中的活性表达及其表达的临床意义。方法:采用端粒重复序列扩增PCR酶联免疫吸附法检测了人胃癌11例,结肠癌14例,肝癌4例,乳腺癌9例及其相应的38例癌旁组织中的端粒酶活性。结果:在38例肿瘤组织中,有31例端粒酶呈阳性(81.6％);38例癌组织中,有8例阳性(21.1％)。癌组织和癌旁组织中端粒酶的表达均与肿瘤临床病理特征无关。癌旁组织存在端粒酶阳性表达的病例,病理上多伴有不典型增生或癌细胞的浸润转移。结论:端粒酶本质上是细胞增殖活性而非肿瘤的分子水平标记物;对癌旁组织中端粒酶阳性的患者加强观察有助于早期发现某些术后再发或复发的肿瘤。
Objective: To investigate the expression of telomerase activity and its clinical significance in human common malignant tumor tissues and adjacent tissues. Methods: Telomerase activity was detected in 11 cases of human gastric cancer, 14 cases of colon cancer, 4 cases of liver cancer, 9 cases of breast cancer and 38 cases of paracancerous tissues by telomeric repeat amplification PCR. . RESULTS: Totally 31 telomerase positive cells (81.6%) were found in 38 tumor tissues, and 8 of 38 cancerous tissues were positive (21.1%). The expression of telomerase in cancer tissue and adjacent tissues was not related to the clinicopathological features of the tumor. Paracancerous tissue adjacent to the presence of positive cases of telomerase, pathologically more with atypical hyperplasia or invasion and metastasis of cancer cells. CONCLUSIONS: Telomerase is essentially a molecular marker of cell proliferation activity, not a tumor marker. Intensive observation of telomerase-positive patients in paracancerous tissues may be helpful in early detection of some recurrent or recurrent tumors.