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目的:分析并比较红车轴草2种主要异黄酮成分鹰嘴豆芽素A和大豆苷元对成骨样细胞MC3T3-E1增殖、分化和矿化的影响。方法:分别采用MTT法、碱性磷酸酶活性测定法、茜素红染色测定矿化结节的方法和ELISA法考察鹰嘴豆芽素A和大豆苷元对MC3T3-E1细胞增殖、分化、矿化以及骨钙素分泌的影响。结果:各浓度鹰嘴豆芽素A和大豆苷元均可促进MC3T3-E1细胞的增殖和分化,且最佳浓度均为1×10~(-7)mol/L;鹰嘴豆芽素A(1×10~(-7)mol/L)处理细胞9、12 d,形成矿化结节数显著高于对照组,大豆苷元(1×10~/(-7)mol/L)处理细胞6、9、12d,形成矿化结节数均显著高于对照组;鹰嘴豆芽素A(1×10~(-7)mol/L)在第6天可显著促进骨钙素的分泌,大豆苷元(1×10~(-7)mol/L)在第3、6、9、12天均可以显著性促进骨钙素的分泌。结论:鹰嘴豆芽素A和大豆苷元均具有促进MC3T3-E1细胞增殖、分化和矿化的作用,且大豆苷元促进成骨分化成熟的活性高于鹰嘴豆芽素A,具有开发为抗骨质疏松新药的潜力。
OBJECTIVE: To analyze and compare the effects of two main isoflavone components, chickpea sp. A and daidzein, on proliferation, differentiation and mineralization of osteoblast-like cells MC3T3-E1. Methods: MTT assay, alkaline phosphatase activity assay, alizarin red staining of mineralized nodules and ELISA method were used to observe the proliferation, differentiation and mineralization of chickpea sp. A and daidzein in MC3T3-E1 cells And the impact of osteocalcin secretion. Results: The chickpea sp. A and daidzein at different concentrations all promoted the proliferation and differentiation of MC3T3-E1 cells, and the optimal concentrations were 1 × 10 -7 mol / × 10 ~ (-7) mol / L) for 9 and 12 days, the number of mineralized nodules was significantly higher than that of the control group. The cells treated with daidzein (1 × 10 ~ (-7) mol / L) , 9,12d, the number of mineralized nodules formed were significantly higher than the control group; chickpea splenic A (1 × 10 ~ (-7) mol / L) on the 6th day can significantly promote the secretion of osteocalcin, Aglycone (1 × 10 ~ (-7) mol / L) could significantly promote the secretion of osteocalcin on the 3rd, 6th, 9th and 12th days. CONCLUSION: Both chickpea sp. A and daidzein can promote the proliferation, differentiation and mineralization of MC3T3-E1 cells, and the activity of daidzein to promote osteogenic differentiation is higher than that of chickpea sp. A, Potential of new drugs for osteoporosis.