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采用不同浓度NaCl(0、100、200、300和500mmol/L)的Hoagland’s培养液处理两种淫羊藿属植物——淫羊藿、箭叶淫羊藿幼苗,处理30d后分别测定其株高、叶表面积、干物质生物量以及根、茎、叶的总黄酮含量,以明确淫羊藿属两种药用植物的抗盐特性。结果显示:(1)盐胁迫对淫羊藿和箭叶淫羊藿的生长均有影响,随着NaCl处理浓度的增加,淫羊藿、箭叶淫羊藿的株高和叶表面积均逐渐显著减小;各器官干物质生物量逐渐降低,但淫羊藿的干物质下降量较小(不显著),且同一处理下淫羊藿的生物量明显小于箭叶淫羊藿。(2)随着NaCl胁迫浓度的增加,除淫羊藿茎的总黄酮含量一直呈下降趋势外,淫羊藿根、叶和箭叶淫羊藿根、茎、叶均呈现在低浓度范围内总黄酮含量增加,且箭叶淫羊藿的含量高于淫羊藿;而在高浓度盐胁迫下,其总黄酮含量均呈下降的趋势,且淫羊藿的含量高于箭叶淫羊藿。研究表明,淫羊藿的抗盐性明显高于箭叶淫羊藿,适于高盐土壤环境种植,但在低盐环境条件下,种植箭叶淫羊藿可显著提高总黄酮产量并提高经济效益。
Treatment of Epimedium and Epimedium sagittatum with different concentrations of NaCl (0, 100, 200, 300, and 500 mmol/L) Hoagland’s medium was performed. The plant height was measured after 30 days of treatment. The leaf surface area, dry matter biomass, and total flavonoid content of roots, stems, and leaves were used to determine the salt resistance of two medicinal plants of Epimedium. The results showed that: (1) Salt stress had an effect on the growth of epimedium and Epimedium sagittatum. With the increase of NaCl concentration, the plant height and leaf surface area of epimedium and Epimedium sagittatum were gradually significant. Decreased; dry matter biomass of various organs gradually decreased, but the dry matter reduction of Epimedium was small (not significant), and the biomass of epimedium under the same treatment was significantly lower than that of Epimedium sagittatum. (2) With the increase of NaCl stress concentration, the content of total flavonoids in Epimedium sinensis leaves has been decreasing trend. In addition, the roots, stems and leaves of Epimedium brevicornum, Epimedium sagittatum, and Epimedium sagittatum are present in a low concentration range. The content of total flavonoids increased, and the content of Epimedium sagittatum was higher than Epimedium; while under high concentration of salt stress, the total flavone content showed a downward trend, and the content of Epimedium was higher than that of Epimedium sagittatum. . Studies have shown that the anti-salinity of Epimedium is significantly higher than that of Epimedium sagittatum and is suitable for high-salt soil environment. However, under low-salt conditions, Epimedium sagittatum can significantly increase total flavonoid production and improve economic efficiency. benefit.