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目的分析中国I型艾滋病病毒(HIV-1)感染婴儿基因亚型的分布情况,以及env区V3环氨基酸序列特征。方法利用巢式聚合酶链反应(nPCR)扩增HIV-1前病毒脱氧核糖核酸(DNA)env区和pol区的基因序列,使用ChromasPro、MEGA 4.0、BioEdit软件进行HIV-1基因分型以及env区V3环氨基酸序列的特征分析。结果对65名HIV-1感染婴儿的100份干血斑样本(DBS)分别进行HIV-1 env区和pol区扩增,最终扩增成功的样本为63份和84份;其中45名感染婴儿的59份DBS两基因区均扩增成功。依据两基因区域均扩增成功的45名感染婴儿样本进行基因分型,结果为:CRF_BC亚型占73.3%(33/45,包括CRF_07-BC 21份,CRF_BC-08 11份),CRF01-AE亚型占20.0%(9/45),B′亚型占4.4%(2/45),G亚型占2.2%(1/45)。对env区扩增成功的63份样本的V3环顶端四肽特征序列进行分析,结果显示存在5种类型,分别为GPGQ、GPGR、GPGS、GLGR和GQGR,以GPGQ为主。53份样本可能使用CCR5作为辅助受体,3份样本可能使用CXCR4作为辅助受体,7份样本不能做出预测。结论婴儿感染的HIV-1亚型呈现多样性分布;HIV-1V3环顶端氨基酸序列以GPGQ为主;大部分婴儿样本可能使用CCR5作为辅助受体。
Objective To analyze the distribution of genotypes of infants infected with HIV-1 in China and the characteristics of V3 loop amino acids in env region. Methods The gene sequences of env region and pol region of HIV-1 pro-virus DNA were amplified by nested polymerase chain reaction (nPCR). The gene sequences of HIV-1 gene were analyzed by using ChromasPro, MEGA 4.0 and BioEdit software. Type and env region V3 loop amino acid sequence characteristics. Results One hundred and seventy blood spot samples (DBS) of 65 HIV-1 infected infants were enrolled in the HIV-1 env region and the pol region respectively. The final successful amplification samples were 63 and 84, of which 45 infants 59 DBS two gene regions were amplified successfully. According to the genotypes of 45 infected infants who were successfully amplified in both genotypes, the CRF_BC subtype accounted for 73.3% (33/45), including 21 CRF_07-BC and 11 CRF_BC-08, CRF01-AE Subtype accounted for 20.0% (9/45), B ’subtype accounted for 4.4% (2/45), G subtype accounted for 2.2% (1/45). The results showed that there are 5 types of GP4Q, GPGR, GPGS, GLGR and GQGR, with GPGQ as the main factor. 53 samples may use CCR5 as a co-receptor, 3 samples may use CXCR4 as a co-receptor, 7 samples can not make a prediction. Conclusion The distribution of HIV-1 subtypes in infants is diverse. The amino acid sequence of the top of HIV-1 V3 ring is GPGQ. Most of the infants may use CCR5 as a co-receptor.