论文部分内容阅读
目的:研究常氧条件下姜黄素对Hep1细胞的增殖及HIF-1α mRNA表达的影响。方法CCK-8法检测常氧条件下不同浓度姜黄素(0、5、10、15、20μmol/L)对肝癌Hep1细胞增殖的影响;用终浓度为15μmol/L姜黄素对体外肝癌细胞系Hep1细胞进行干预,流式细胞术分析Hep1细胞凋亡的变化;常氧条件下不同浓度姜黄素(0、5、10、15、20μmol/L)对肝癌Hep1细胞作用24h后,RT-PCR检测Hep1细胞HIF-1α mRNA表达水平的变化。结果:5、10、15、20μmol/L的姜黄素在作用1周后,呈浓度依赖性抑制肝癌Hep1细胞增殖;流式分析显示15μmol/L的姜黄素使Hep1细胞凋亡率增高,差异具有统计学意义(P<0.05);与空白对照组相比,10μmol/L姜黄素处理组的HIF-1α mRNA的表达水平升高,差异具有统计学意义(P<0.05),15、20μmol/L姜黄素处理组的HIF-1α mRNA的表达水平升高更为显著(P<0.01)。结论:常氧条件下,姜黄素呈浓度依赖性抑制肝癌Hep1细胞的增殖,促进Hep1细胞的凋亡和HIF-1α mRNA的表达,常氧条件下姜黄素抑制肝癌Hep1细胞增殖的机制可能与缺氧条件下姜黄素的抑瘤机制有所不同。
Objective: To study the effect of curcumin on the proliferation of Hep1 cells and the expression of HIF-1α mRNA under normoxia. Methods The effects of curcumin (0, 5, 10, 15 and 20μmol / L) on the proliferation of Hep1 cells were detected by CCK-8 assay. The effect of curcumin at a final concentration of 15μmol / L on Hep1 The effect of curcumin (0, 5, 10, 15, 20μmol / L) on Hep1 cells was detected by flow cytometry. The effect of Hep1 Changes of cell HIF-1α mRNA expression. Results: Curcumin at 5, 10, 15 and 20μmol / L inhibited the proliferation of Hep1 cells in a concentration-dependent manner for 1 week. Flow cytometry showed that curcumin at 15μmol / L increased the apoptotic rate of Hep1 cells, (P <0.05). Compared with the blank control group, the expression of HIF-1α mRNA in the 10μmol / L curcumin treatment group was significantly increased (P <0.05) The expression level of HIF-1α mRNA in curcumin group increased more significantly (P <0.01). CONCLUSION: Under normoxic conditions, curcumin inhibits the proliferation of Hep1 hepatocarcinoma cells in a concentration-dependent manner and promotes the apoptosis of Hep1 cells and the expression of HIF-1α mRNA in a concentration-dependent manner. Curcumin inhibits the proliferation of hepatocellular carcinoma Hep1 cells under normoxia, Curcumin oxygen inhibition mechanism is different.