目的协作标定第二批人凝血酶国家标准品。方法用世界卫生组织首批α 凝血酶国际标准品 (批号 :89/ 5 88)为标准 ,采用纤维蛋白原凝固法标定。并将标准品分别于 2 5℃、37℃、4 5℃保存 6个月 ,进行稳定性考查。结果第二批人凝血酶国家标准品的效价为 12 7IU/支 ,稳定性良好 ,其他项目符合国家标准品要求。结论已成功研制第二批人凝酶国家标准品 ,可以取代首批人凝血酶国家标准品。 Objective To coordinate the second batch of human thrombin standard. Methods The first WHO international standard α-thrombin (batch number: 89/5 88) as the standard, using fibrinogen coagulation method. The standards were stored at 25 ℃, 37 ℃ and 45 ℃ for 6 months, respectively, for stability test. Results The second batch of human thrombin national standard potency of 12 7IU / support, good stability, other projects in line with national standards. Conclusion The second batch of human coagulase national standards has been successfully developed, which can replace the first national standards of human thrombin.