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白细胞介素3(Interleukin-3;IL-3)是在ConA、异种抗原刺激下由小鼠淋巴细胞产生的.Ihle 等报告它具有诱导裸鼠脾细胞20α-羟类固醇脱氢酶活性(SDH)的作用,尚有刺激肥大细胞增殖、粒-巨噬细胞的 CSF 活性,以及支持小鼠前 B 细胞增殖等方面的作用。作者用5-氟脲嘧啶(5-FU)处理的小鼠脾细胞采用 lscore 的甲基纤维素培养法研究了 IL-3对血液干细胞增殖分化的作用。在50—400u/ml IL-3存在下,5-FU 处理的小鼠脾细胞培养4—16天形成未分化细胞构成的集落,
Interleukin-3 (IL-3) is produced by mouse lymphocytes stimulated by ConA and xenogeneic antigen and Ile et al. Report that it induces 20α-hydroxysteroid dehydrogenase activity (SDH) in splenocytes of nude mice The role of stimulation of mast cells, CSF activity of granulocyte macrophages, as well as to support the role of mouse pre-B cell proliferation. The effect of IL-3 on the proliferation and differentiation of hematopoietic stem cells was investigated by methyl cellulose culture of lscore in mouse splenocytes treated with 5-fluorouracil (5-FU). 5-FU-treated mouse spleen cells were cultured for 4-16 days in the presence of 50-400u / ml IL-3 to form colonies of undifferentiated cells,