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以ＨＣＶＮＳ３区单克隆抗体、ＬＳＡＢ法检测了１１６例各类肝病肝组织（其中肝细胞癌５２例，肝炎后肝硬化１６例，慢性肝炎４０例，体质性黄疸８例）及６份正常肝组织（作为对照）中ＨＣＶ抗原的表达状况，结果表明，ＨＣＶ抗原在肝细胞癌、肝炎后肝硬化及慢性肝炎中检出率分别为１３．５％（７／５２）、１２．５％（２／１６）、１０％（４／４０），而在体质性黄疸及正常肝组织中未检出ＨＣＶ抗原。ＨＣＶ抗原可见于癌细胞或癌周肝细胞的胞浆或胞核中，实验结果支持ＨＣＶ与肝细胞癌的关联，ＨＣＶ感染对促使肝病的加重也具有一定影响。肝细胞癌中，胞核型ＨＣＶ抗原表达较多见。癌周组织中ＨＣＶ抗原检出率高于癌组织，提示ＨＣＶ可能主要作用于癌前病变，在肝细胞癌发生的初始阶段起作用，促使正常细胞恶性化。
A total of 116 cases of liver diseases including hepatocellular carcinoma (HCC), 16 cases of posthepatitic cirrhosis, 40 cases of chronic hepatitis and 8 cases of constitutional jaundice were detected by LSAB method with HCVNS3 monoclonal antibody and 6 normal liver tissues (As a control). The results showed that the detection rates of HCV antigen in hepatocellular carcinoma, posthepatitic cirrhosis and chronic hepatitis were 13.5% (7/52), 12.5% (2) / 16) and 10% (4/40), respectively. However, no HCV antigen was detected in physical jaundice and normal liver tissue. HCV antigen can be seen in the cytoplasm or nucleus of cancer cells or peritumor hepatocytes. The experimental results support the association between HCV and hepatocellular carcinoma, and HCV infection has an impact on exacerbating liver disease. Hepatocellular carcinoma, nuclear type HCV antigen expression more common. The detection rate of HCV antigen in peri-cancerous tissues is higher than that in cancerous tissues, suggesting that HCV may mainly act on precancerous lesions and play an important role in the initial stage of hepatocellular carcinoma and promote the malignant transformation of normal cells.