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目的探讨异氟烷和七氟烷对老年小鼠认知功能的影响及与胆碱能系统的关系。方法健康雌性18个月龄C57BL/6J老年小鼠30只,分为3组,每组10只。1.2%异氟烷(异氟烷组)或2.6%七氟烷(七氟烷组)麻醉6h(同时吸入30%氧气和70%氮气混合气体),未麻醉组为对照组。麻醉后2周行Morris水迷宫实验检测认知功能。水迷宫实验结束后取脑组织测定乙酰胆碱酯酶(AchE)和乙酰胆碱转移酶(ChAT)的活性和含量。结果与对照组比较,异氟烷组潜伏期和游泳路程均延长(P<0.05),小鼠脑组织内ChAT含量降低;七氟烷组潜伏期和游泳路程均无显著变化(P>0.05),AchE和ChAT的活性及含量均无显著变化。3组之间目标象限的时间和路程百分比均无显著性差异(P>0.05)。结论异氟烷麻醉后老年小鼠出现术后认知功能障碍(POCD),可能与ChAT含量减少有关。七氟烷麻醉后老年小鼠未出现POCD,且AchE和ChAT的活性及含量均无变化。
Objective To investigate the effect of isoflurane and sevoflurane on cognitive function in elderly mice and its relationship with cholinergic system. Methods Thirty healthy female 18-month-old C57BL / 6J aged mice were divided into 3 groups with 10 in each. 1.2% isoflurane (isoflurane group) or 2.6% sevoflurane (sevoflurane group) anesthesia for 6h (inhalation of 30% oxygen and 70% nitrogen mixed gas), not anesthesia group as the control group. Two weeks after anesthesia, Morris water maze test was performed to detect cognitive function. After the water maze test, the brain tissue was taken to measure the activity and content of acetylcholinesterase (AchE) and acetylcholinesterase (ChAT). Results Compared with the control group, the latency and swimming distance of isoflurane group were prolonged (P <0.05), while the content of ChAT in brain tissue of mice was decreased. There was no significant change in latency and swimming distance in sevoflurane group (P> 0.05) And ChAT activity and content were not significantly changed. There was no significant difference in time and distance between the three groups (P> 0.05). Conclusion Postoperative cognitive impairment (POCD) in aged mice after isoflurane anesthesia may be related to the decrease of ChAT content. Sevoflurane anesthesia in elderly mice did not appear POCD, and AchE and ChAT activity and content were not changed.