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研究了发根农杆菌R16 0 1对野葛 (Puerarialobata)进行遗传转化的影响因素 .结果表明 :叶片是转化效率较高的外植体 ,经发根农杆菌R16 0 1感染 12d后可获得毛状根 ;降低YEB活化液的pH值 ,或以肌醇 (10g L)代替酵母提取成分 ,可明显提高菌种的致根性 ;叶片预培养 2～ 3d可提前 3～ 4d出根 .表 5参 14
The effects of Agrobacterium rhizogenes R16 0 1 on the genetic transformation of Puerarialobata were studied.The results showed that the leaf explants with high transformation efficiency could be obtained after 12 days of infection by Agrobacterium rhizogenes R16 0 1 Rhizome root; to reduce the pH value of YEB activation solution, or inositol (10g L) instead of yeast extract can significantly improve the rooting of species; leaf pre-culture 2 ~ 3d 3 ~ 4d early rooting. 14