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目的建立活骨胶囊的质量标准。方法采用TLC法对活骨胶囊中当归、骨碎补、葛根、何首乌进行定性鉴别,采用HPLC法测定制剂中2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量。结果 TLC分离清晰,阴性无干扰;2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在0.129~2.064μg范围内呈良好的线性关系(r=0.9999),平均回收率为100.15%,RSD为0.76%(n=6)。结论该方法简便、灵敏、准确、重复性好,可作为活骨胶囊的质量控制标准。
Objective To establish the quality standard of live bone capsule. Methods The TLC method was used to qualitatively identify Angelica, Rhizoma Drynariae, Radix Puerariae and Radix Polygonum multiflorum. The content of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D - glucoside content. Results TLC was clear and negative without interference. The linearity of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside in the range of 0.129-2.064μg was good (r = 0.9999 ), With an average recovery of 100.15% and a RSD of 0.76% (n = 6). Conclusion The method is simple, sensitive, accurate and reproducible, and can be used as a quality control standard for Huogu capsule.