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目的探讨胃癌组织中食管癌相关基因4(Esophageal cancer-related gene 4,ECRG4)启动子区的甲基化情况及其临床意义。方法应用甲基化特异性PCR(Methylation specific-PCR,MSP-PCR)法,检测49份胃癌组织、30份癌旁组织和15份正常组织标本中ECRG4基因启动子区的甲基化情况,并分析其与临床病理特征之间的相关性。结果胃癌组织[69.4%(34/49)]和癌旁组织[53.3%(16/30)]ECRG4基因甲基化检出率均明显高于正常组织[6.7%(1/15)](P<0.01);Ⅲ+Ⅳ期ECRG4基因甲基化检出率[80%(24/30)]明显高于Ⅰ+Ⅱ期[52.6%(10/19)](P<0.05),表明ECRG4基因甲基化检出率与病理分期相关,而与患者年龄、性别及淋巴结转移无相关性(P>0.05)。结论 ECRG4基因启动子区异常甲基化与胃癌的发生发展密切相关,可作为胃癌早期辅助诊断的分子标志物之一。
Objective To investigate the methylation status of the promoter region of esophageal cancer-related gene 4 (ECRG4) in gastric cancer and its clinical significance. Methods Methylation-specific PCR (MSP-PCR) was used to detect the methylation status of ECRG4 promoter in 49 gastric cancer tissues, 30 adjacent tissues and 15 normal tissues. Analyze its correlation with clinicopathological features. Results The methylation rates of ECRG4 gene in gastric cancer tissues (69.4%, 34/49) and adjacent tissues (53.3%, 16/30) were significantly higher than those in normal tissues [6.7% (1/15)] <0.01). The detection rate of methylation of ECRG4 gene in stage Ⅲ + Ⅳ was significantly higher than that in stage Ⅰ + Ⅱ [52.6% (10/19)] (80% (24/30)] (P <0.05) The detection rate of methylation was correlated with pathological stage, but not with age, gender and lymph node metastasis (P> 0.05). Conclusion Aberrant methylation of ECRG4 promoter region is closely related to the occurrence and development of gastric cancer and may be used as one of the molecular markers for early diagnosis of gastric cancer.