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目的:探讨二烯丙基二硫(Diallyl disulfide)诱导白血病K562细胞凋亡的作用及其机制。方法:采用吖啶橙/溴化乙啶(AO/EB)染色法观察细胞凋亡形态学变化;DNA琼脂糖凝胶电泳测定DNA梯度带;RT-PCR法检测BAG-1、BAX基因的mRNA表达变化。结果:DADS可诱导K562细胞凋亡。其对K562细胞的凋亡效用与药物浓度、有明显依赖关系;DNA琼脂糖凝胶电泳示:40mg/LDADS作用K562细胞48小时后能够产生明显的梯形电泳图谱(DNA ladder):DADS作用48h后,BAX mRNA表达水平较对照组上调;BAG-1 mRNA较对照组下调(差异具有统计学意义,P<0.05)。结论:DADS能够诱导K562细胞凋亡,其凋亡机制可能与上调BAX,下调BAG-1有关。
Objective: To investigate the effect and mechanism of Diallyl disulfide on apoptosis of leukemia K562 cells. Methods: Apoptosis morphological changes were observed by acridine orange / ethidium bromide (AO / EB) staining; DNA gradient was detected by DNA agarose gel electrophoresis; mRNA of BAG-1 and BAX was detected by RT-PCR Change of expression Results: DADS can induce apoptosis of K562 cells. The apoptosis of K562 cells and drug concentration significantly dependent; DNA agarose gel electrophoresis showed: 40mg / LDADS K562 cells after 48 hours to produce a significant ladder pattern (DNA ladder): DADS effect after 48h , BAX mRNA expression was up-regulated compared with the control group; BAG-1 mRNA was down-regulated compared with the control group (the difference was statistically significant, P <0.05). Conclusion: DADS can induce apoptosis in K562 cells. The mechanism of apoptosis may be related to up-regulation of BAX and down-regulation of BAG-1.