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目的对2例微柱凝胶卡漏检B_w03亚型标本血型血清学特点及其分子遗传背景进行系统分析,以期为临床安全输血提供帮助。方法分别应用试管法和微柱凝胶卡法进行血型正反定型;应用PCR-SSP方法对ABO血型基因进行初步分型;并进一步应用直接测序和单体型序列分析法对ABO基因第6、7外显子进行分析以确定其基因型。结果标本1:试管法结果疑似B亚型,凝胶卡结果正定为O型反定为B型,基因检测结果为B_w03。标本2:试管法结果疑似AB亚型,凝胶卡结果正定为A型反定为AB型,基因检测结果为AB_w03。结论微柱凝胶卡能漏检B_w03亚型,应采用多种方法进行血型鉴定以减少临床血型鉴定错误及相关输血反应的发生。
Objective To systematically analyze the blood group serological features and molecular genetic background of 2 subtype B_w03 samples of micro-column gel card, in order to provide help for clinical transfusion safety. Methods The blood type positive and negative typing were respectively performed by tube method and microcolumn gel card method. The genotypes of ABO blood group were preliminarily genotyped by PCR-SSP method. The direct sequencing and haplotype sequence analysis were used to analyze the ABO genotypes 6, 7 exons were analyzed to determine their genotype. Results Specimen 1: The test tube method suspected subtype B, the gel card results positive O-type reversed to B-type, gene test results for B_w03. Specimen 2: The test tube method was suspicious for AB subtype, the gel card result was positive for type A and negative for type AB, and the gene test result was AB_w03. Conclusion Micro-column gel cards can detect subtype B_w03. Blood samples should be identified by various methods to reduce the errors of clinical blood-type identification and the related transfusion reaction.