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利用MTT法测定了商陆皂甙辛 (EH )对ConA诱导的小鼠脾淋巴细胞IL 3和IL 6活性的影响 ,采用地高辛标记斑点杂交法测定了IL 3mRNA和IL 6mRNA的表达水平。结果显示EH在 1 0～ 1 0 0 μg/ml浓度范围内能增强ConA诱导的小鼠脾淋巴细胞IL 3和IL 6活性 ,伴随mRNA水平的上升。提示EH通过提高IL 3和IL 6基因转录水平 ,从而使其产物活性增强 ,是其免疫调节和提高造血功能的分子机制之一
The effect of phytolactoxin (EH) on the activity of IL 3 and IL 6 in spleen lymphocytes of mice induced by ConA was measured by MTT assay. The expression levels of IL 3 mRNA and IL 6 mRNA were measured by digoxigenin dot blot hybridization. The results showed that EH enhanced the ConA-induced IL 3 and IL 6 activity in mouse spleen lymphocytes in the concentration range of 10 to 100 μg/ml, with an increase in mRNA levels. It is suggested that EH enhances the activity of IL 3 and IL 6 gene by increasing the transcription level of IL 3 and IL 6, which is one of the molecular mechanisms of immune regulation and hematopoiesis.