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用茜素络合物和盐酸四环素溶液分别对胭脂鱼(Myxocyprinusasiaticus)仔、稚鱼浸泡标记,结果表明,100~200mg/L的茜素络合物溶液浸泡24h对胭脂鱼仔、稚鱼耳石有很好的标记效果。相同浸泡浓度下,随着日龄的增长,耳石上的荧光反应强度降低。三对耳石中,微耳石和矢耳石对茜素络合物较敏感,星耳石敏感性则较低。盐酸四环素溶液对仔、稚鱼耳石的标记效果很差,浸泡液浓度为100mg/L和120mg/L时,仅能在微耳石上检测到较弱的荧光标记,而150mg/L及以上浓度的浸泡液对稚鱼有较高的致死作用。因此,茜素络合物是对胭脂鱼早期鱼苗进行化学标记比较合适的荧光物质,而盐酸四环素不适于标记该鱼的耳石。在对标记鱼进行荧光检测时,矢耳石和微耳石是适合的材料。
The alizarin complex and tetracycline hydrochloride solution were respectively labeled with Myxocyprinus asiaticus and juvenile soaked in alizarin complex solution at 100 ~ 200 mg / L for 24 h. Good markup effect. With the same soaking concentration, with the increasing of age, the fluorescence intensity of otolith decreased. Among the three pairs of otoliths, otolith and otolith are more sensitive to alizarin complex and less sensitive to otolith. The tetracycline hydrochloride solution showed poor labeling effect on otoliths and juvenile otoliths. Only a weak fluorescent marker could be detected on the microplate ossicles when the soaking solution concentration was 100mg / L and 120mg / L, while the concentration of 150mg / L and above The soaking liquid has a higher lethal effect on juveniles. Therefore, alizarin complex is a suitable fluorescent substance for chemical labeling of early fry fish, while tetracycline hydrochloride is not suitable for labeling the otolith of fish. Agar and otoliths are suitable materials for fluorescent detection of labeled fish.