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目的:探讨Survivin、p63在喉鳞状细胞癌(LSCC)组织中的表达及与LSCC发生、发展的关系和生物学意义。方法:应用原位分子杂交方法,检测SurvivinmRNA、p63mRNA在47例LSCC组织、癌旁组织及14例正常喉组织中的表达。结果:①SurvivinmRNA在正常喉组织中不表达;在LSCC组织、癌旁组织中的阳性表达率分别为59.6%(28/47)、25.5%(12/47),其差异有统计学意义(P<0.05);组织分化程度越低、TNM分期越晚、淋巴结转移率越高,则SurvivinmRNA表达越强(均P<0.05)。②p63mRNA在LSCC组织、癌旁组织中的阳性表达率分别为91.5%(43/47)、93.6%(44/47),分别与正常喉组织(57.1%)比较,差异有统计学意义(P<0.05);p63mRNA与喉癌临床病理特征无关。结论:Survivin在LSCC中表达上调,提示其通过抑制细胞凋亡在LSCC的发生、发展过程中起重要作用,可能成为LSCC基因治疗的新靶点;其高表达预示肿瘤有较高的侵袭性和不良预后。p63对LSCC生物学行为的影响较小。
Objective: To investigate the expression of Survivin and p63 in laryngeal squamous cell carcinoma (LSCC) and its relationship with the occurrence and development of LSCC and its biological significance. Methods: In situ hybridization was used to detect the expression of Survivin mRNA and p63 mRNA in 47 cases of LSCC, adjacent normal tissues and 14 cases of normal laryngeal tissues. Results: ①Survivin mRNA was not expressed in normal laryngeal tissues. The positive rates of Survivin mRNA in LSCC tissues and paracancer tissues were 59.6% (28/47) and 25.5% (12/47), respectively, with significant difference (P < 0.05). The lower the degree of tissue differentiation, the later TNM stage, the higher the rate of lymph node metastasis, the stronger the expression of Survivin mRNA (all P <0.05). The positive rates of p63 mRNA in LSCC tissues and paracancer tissues were 91.5% (43/47) and 93.6% (44/47), respectively, which were significantly different from those of normal laryngeal tissues (57.1%, P < 0.05). P63 mRNA was not associated with clinicopathological features of laryngeal carcinoma. CONCLUSIONS: Survivin is up-regulated in LSCC, suggesting that it may play an important role in the genesis and progression of LSCC by inhibiting apoptosis. It may be a new target for LSCC gene therapy. The high expression of Survivin indicates that the tumor has higher invasiveness and Poor prognosis. p63 has little effect on the biological behavior of LSCC.