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目的应用相关软件和在线网络分析Em95的蛋白二级结构,预测B细胞表位和T细胞表位的联合表位。方法采用DNAStar软件和在线网络IEDB、SYFPEITHI及Propred等对Em95的B细胞表位和T细胞表位进行预测,寻找B细胞和T细胞表位共同的区域。结果 Em95存在潜在的抗原表位,其中B细胞表位区域为10-19、43-48、52-59、78-82、92-100和110-115;分值高的T细胞表位区域为32-40、51-60、82-98、108-116和126-138。B细胞和T细胞共同的表位区域为52-59、92-98和110-115。结论运用生物信息学方法确定Em95的3个T-B细胞联合表位存在于5个T细胞抗原表位和6个B细胞抗原表位中存在区域,对进一步研究Em95的抗原性和研发优势表位疫苗具有重要意义。
Objective To analyze the protein secondary structure of Em95 and predict the combined epitopes of B cell epitopes and T cell epitopes by using software and online network. Methods DNAStar software and on-line networks IEDB, SYFPEITHI and Propred were used to predict the B cell epitopes and T cell epitopes of Em95 in order to find common regions of B cells and T cell epitopes. Results There was a potential antigen epitope in Em95, in which the B cell epitope regions were 10-19, 43-48, 52-59, 78-82, 92-100 and 110-115; the high T cell epitope region was 32-40, 51-60, 82-98, 108-116 and 126-138. The common epitope regions for B cells and T cells are 52-59, 92-98 and 110-115. Conclusion The bioinformatics method was used to determine the presence of three TB cell epitopes in Em95 in the presence of five T cell epitopes and six B cell epitopes. To further investigate the antigenicity of Em95 and to develop a superior epitope vaccine It is of great significance.