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将SPF级昆明小鼠按体重随机分为5组,分别为0(阴性对照)、500、1 000、2 000 mg/kg六苄基六氮杂异伍兹烷(HBIW)染毒组以及30 mg/kg环磷酰胺组。采用小鼠骨髓嗜多染细胞微核试验检测嗜多染红细胞(PCE)微核率和PCE与正染红细胞(NCE)比值,采用骨髓细胞染色体畸变试验检测染色体数目及结构畸变率。结果显示,与阴性对照组相比,各剂量染毒组小鼠PCE微核率、PCE/NCE、染色体数目及结构畸变率差异均无统计学意义。提示在本实验剂量范围内,HBIW对小鼠骨髓细胞无致突变效应。
The SPF Kunming mice were randomly divided into five groups according to body weight, respectively, as 0 (negative control), 500, 1 000, 2 000 mg / kg HBIW exposure groups and 30 mg / kg cyclophosphamide group. The micronucleus rate of PCE and the ratio of PCE to NCE were detected by mouse bone marrow polychrome micronucleus test. Chromosome number and structure aberration rate were detected by chromosome aberration test of bone marrow cells. The results showed that compared with the negative control group, there was no significant difference in PCE micronucleus rate, PCE / NCE, chromosome number and structural aberration rate in mice exposed to different doses. It is suggested that HBIW has no mutagenic effect on mouse bone marrow cells within the experimental dose range.