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目的利用超高倍显微系统对患儿咽拭子进行肺炎支原体(MP)活体检测,探讨小儿MP早期感染的诊断新方法及其价值。方法用ACT-2000超高倍显微镜,对522例儿科门诊和病房收治的高度疑为MP感染患儿行咽拭子镜检,同时,采集患儿静脉血检测血清肺炎支原体抗体(MP-IgM)。结果咽拭子检查MP感染阳性者402例(77.0%),血清学检查阳性者376例(72.0%)。随着患儿病程时间的推移,两者阳性率有所差异。结论应用超高倍显微系统对咽拭子行MP检查,能早期、快速诊断小儿呼吸道MP感染,为临床及时、快速提供准确的诊断依据。
Objective To detect Mycoplasma pneumoniae (MP) in children with throat swab using ultra-high magnification microscopy to explore a new method of diagnosis and early diagnosis of MP infection in children. Methods A total of 522 pediatric outpatients and wards were enrolled in this study. Throat swab was used for microscopy in children with suspected MP infection. Serum Mycoplasma pneumoniae antibody (MP-IgM) was detected in venous blood by ACT-2000 microscope. Results Throat swabs were positive for MP infection in 402 patients (77.0%) and serologically positive for 376 patients (72.0%). With the passage of time duration of children, the positive rate of the two is different. Conclusions MP examination of throat swab using ultra-high magnification microscopy can diagnose MP infection of the respiratory tract in children early and quickly, providing an accurate diagnostic basis for clinical timely and rapid diagnosis.