论文部分内容阅读
目的：观察长时间给予μ阿片受体肽类激动剂DAGO及非肽类激动剂OMF对μ受体基因表达的影响，并比较这两者作用的差异性。方法：采用反转录-聚合酶链反应（RT－PCR）方法。结果：（1）DAGO使μ受体mRNA水平显著降低，作用持续48h；而OMF作用只持续24h；（2）DAGO在10－8mol·L-1时即可使μ受体的mRNA水平显著降低，而OMF在10－7mol·L-1时才有效。结论：长时间给予肽类及非肽类μ激动剂均可以显著抑制μ受体的基因表达；DAGO作用较强，时程较长。
Objective: To observe the effect of prolonged administration of μ opioid receptor peptide agonist DAGO and non-peptide agonist OMF on μ receptor gene expression, and to compare the differences between the two. Methods: Reverse transcription - polymerase chain reaction (RT-PCR) was used. Results: (1) DAGO significantly reduced μ receptor mRNA level for 48 h, while OMF only lasted for 24 h. (2) DAGO at 10-8 mol·L-1 significantly decreased μ-receptor mRNA levels , While OMF was only effective at 10-7mol·L-1. CONCLUSION: Long-time administration of peptides and non-peptide μ agonists can significantly inhibit μ receptor gene expression; DAGO stronger role, longer duration.