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模仿雄性锯缘青蟹第一腹肢,根据其在交配中的作用机制,采用常规滴管为材料,利用酒精灯灼热拉制一个前端弯曲、侧扁、管径大小接近第一腹肢的人工交接器.以无钙离子的人工海水作为青蟹人工授精的缓冲液,缓冲液中加入适量庆大霉素.首次对青蟹采用类似硬壳交配的方式,将成熟雄蟹贮精囊内的精荚和精液成功移植至雌蟹纳精囊中.结果表明精荚的人工移植不会破坏成熟雌蟹纳精囊的内环境,其内精子具正常受精能力.未交配雌青蟹精荚的人工移植没有正常产卵,但性腺得以促进发育,并能进行正常的生殖脱皮.
Mimicking the first abdominal limb of male marsh margin crab, according to its mechanism of action in mating, the conventional dropper was used as the material to make a frontal bent, flattened and close to the first abdomen Artificial seawater without calcium ions as artificial insemination buffer of blue crab, buffer adding appropriate amount of gentamicin.For the first time on the blue crab with a similar way of hard shell mating, And semen were successfully transplanted into the female crab spermatheca.The results showed that the artificial transplanting of the spermatophaga did not destroy the internal environment of the mature female crab spermatheca and the inner spermatozoa had the normal ability of fertilization.The artificial transplants of the unmated males were not normal Spawning, but the gonads can promote development, and normal reproductive peeling.