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目的探讨灵芝多糖(GLP)对失血性休克复苏时肺损伤的保护作用。方法复制家兔失血性休克再灌注(SH-R)复苏模型,随机分成假手术组(S组)、生理盐水再灌注复苏组(NS组)和质量分数为1%的GLP再灌注复苏组(LS组)。于再灌注复苏90min时快速处死动物,观察肺组织一氧化氮(NO)含量和一氧化氮合酶(NOS)活性与肺组织损伤性变化的关系。结果NS和LS组肺组织NOS活性、NO含量均明显低于S组,但LS组明显高于NS组(P<0.05);NS和LS组肺组织湿/干比(W/D)、损伤肺泡百分率(IAR)和凋亡细胞百分数、支气管肺泡灌洗液(BALF)中白细胞计数和肺通透指数(PPI)明显高于S组,而LS组明显低于NS组(P<0.05);病理显示:NS组肺损伤明显,LS组肺损伤不明显。结论GLP可通过激活NOS、增加NO含量、减少多形核白细胞(PMN)在肺部的集聚而对SH-R肺损伤起保护作用。
Objective To investigate the protective effect of Ganoderma lucidum polysaccharide (GLP) on lung injury during hemorrhagic shock resuscitation. METHODS: Rabbit models of hemorrhagic shock and reperfusion (SH-R) resuscitation were performed and randomly divided into sham-operated group (S group), saline reperfusion group (NS group) and GLP reperfusion group (1%). LS group). The animals were quickly sacrificed at 90 min after reperfusion, and the relationship between the content of nitric oxide (NO) and the activity of nitric oxide synthase (NOS) and the changes of lung tissue injury was observed. Results The NOS activity and NO content in the lung tissue of NS and LS groups were significantly lower than those in S group, but the LS group was significantly higher than that in NS group (P<0.05); the wet/dry ratio (W/D) and damage of lung tissue in NS and LS groups. Percentage of alveolar (IAR) and percentage of apoptotic cells, white blood cell count and lung permeability index (PPI) in bronchoalveolar lavage fluid (BALF) were significantly higher than those in S group, while those in LS group were significantly lower than those in NS group (P<0.05); Pathology showed that lung injury was significant in the NS group, and no lung injury was observed in the LS group. Conclusion GLP can protect against SH-R lung injury by activating NOS, increasing NO content, and reducing the accumulation of polymorphonuclear leukocytes (PMN) in the lungs.