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AIM:To investigate KAI1 gene expression in the progressionof human colonic carcinoma and its clinical significances.METHODS:KAI1 expression was detected by in situhybridization and immunohistochemistry in the 4 establishedcell lines of colorectal carcinoma with different metastaticpotentials,and in 80 specimens of colonic carcinoma,21colonic carcinoma specimens with lymphatic metastasisand 20 controls of normal colonic mucosa.RESULTS:The expressions of KAI1 in HT29 and SW480cell lines were higher than those in LoVo and SW620.Theexpression of KAI1 gene was significantly higher in colorectalcarcinoma compared with normal colonic mucosa andlymphatic metastasis(χ~2=46.838,P<0.01).The expressionof KAI1 gene had no relationship with histological grade.The KAI1 expressions in Dukes A and B carcinoma werehigher at both mRNA and protein levels compared to DukesC carcinoma(χ~2=16.061,P<0.05).The expression of KAI1in colonic carcinoma specimens with lymphatic metastasiswas almost lost.The results of in situ hybridization werein concordance with immunohistochemistry.CONCLUSION:KAI1 is highly related to the metastasis ofcolonic carcinoma and may be a useful indicator of metastasisin colonic carcinoma.
AIM: To investigate KAI gene expression in the progression of human colonic carcinoma and its clinical significances. METHODS: KAI1 expression was detected by in situ hybridization and immunohistochemistry in the 4 established cell lines of colorectal carcinoma with different metastatic potentials, and in 80 specimens of colonic carcinoma, 21colonic carcinoma specimens with lymphatic metastasis and 20 controls of normal colonic mucosa.RESULTS: The expressions of KAI1 in HT29 and SW480 cells lines were higher than those in LoVo and SW620.Theexpression of KAI1 gene was significantly higher in colorectalcarcinoma compared with normal colonic mucosa andlymphatic metastasis (χ ~ 2 = 46.838, P <0.01). The expression of KAI1 gene had no relationship with histological grade. The KAI1 expressions in Dukes A and B carcinoma were both mRNA and protein levels compared to DukesC carcinoma (χ ~ 2 = 16.061, P < 0.05). The expression of KAI1in colonic carcinoma specimens with lymphatic metastasis was almost lost. The resul ts of in situ hybridization were in concordance with immunohistochemistry. CONCLUSION: KAI1 is highly related to the metastasis ofcolonic carcinoma and may be a useful indicator of metastasis in colonic carcinoma.